Figure 3.

Sensitivity of parental (naïve) and Toca 511-infected tumor cell lines to 5-FC and 5-FU. Cell viability was determined in vitro by an MTS assay. Cell lines that had been maximally transduced with Toca 511 vector (or nontransduced parental cells prepared in parallel) were seeded, in quadruplicate, at 1000 cells/well in 96-well plates. The LD₅₀ was determined by MTS assay 6 days after treatment with various concentrations of 5-FC or 5-FU. LD₅₀ values in μg/ml for 5-FC or 5-FU are listed along the y axis. Numerical LD₅₀ values in μg/ml are listed in the embedded table for the transduced and naïve tumor cells treated with 5-FC or 5-FU. This figure is a representative experiment of two or more individual experiments. 5-FC, 5-fluorocytosine; 5-FU, 5-fluorouracil.