Figure 6.

Correlation between yCD protein levels and conversion of 5-FC to 5-FU in Toca 511-infected tumors. Tumor cells that had been maximally transduced with Toca 511 vector were cultured with different concentrations of 5-FC for 24 hr, and intra- and extracellular 5-FU were measured by LC-MS. 5-FC conversion to 5-FU varied up to 10-fold across cell lines, but strongly correlated with yCD protein levels measured by Western blot (Pearson r = 0.82). Data illustrate the 24 hr time point at 66.6 μg/ml 5-FC, but are representative of all time points and 5-FC concentrations measured.