Figure 2. Genotoxic effect of PAM prepared following increasing exposure duration (Texp = 60 s, 120 s and 240 s).

MCTS were transferred in plasma-activated medium after 24 h or 48 h, and fixed 4 h later. Immunodetection of the phosphorylated from of histone H2AX (phospho-H2AX, green) on 5 μm cryosections of control or treated spheroids. DAPI (blue) correspond to the Nuclei. Data shown are representative images from four independent experiments. Scale bar, 100 μm.