Figure 3. Growth inhibition and genotoxic effects of PAM in the presence of catalase.

(a) Variation of the relative volume of HCT116 spheroids grown in plasma-activated media in the presence of catalase (30 μg.mL⁻¹). Spheroids were cultured during five days in DMEM and subjected to plasma-conditioned medium at day 0 for several transfer time Ttrans. Data are shown as means ± SD from four independent experiments. (b) Genotoxic effect of PAM detected 4 h after transfer time of MCTS in PAM. Immuno-detection of the phosphorylation of the histone variant H2AX (phospho-H2AX, green) on 5 μm cryosections of control or treated spheroids. DAPI (blue) correspond to the Nuclei. Data shown are representative images from four independent experiments. Scale bar, 100 μm.