Figure 4. Genotoxic effect of PAM prepared of with free-radical scavengers.

Immunodetection of DNA damage using antibodies against the phosphorylated form of the histone variant H2AX (phospho-H2AX, green) on 5 μm cryosections from control or treated spheroids fixed 4 h after transfer of MCTS in plasma-activated medium (Texp = 120 s and Ttrans 1 hour). DAPI (blue) correspond to the Nuclei. Data shown are representative images from four independent experiments. Scale bar, 100 μm.