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. 2016 Feb 22;6:21598. doi: 10.1038/srep21598

Figure 2. The regulation of circadian miRNA primary transcripts by circadian regulators.

Figure 2

(a) circadian regulator binging sites are enriched on the promoters of the circadian oscillating miRNA primary transcripts comparing to all miRNA primary transcripts. Enrichment p-values were calculated by proportion test. (b) BMAL1 ChIP-PCR showed that the promoter regions of pri-mir-23b~27b~24-1, pri-mir-378, and pri-mir-101a are bound by BMAL1/CLOCK. Gapdh is used as the negative control, while Dbp is used as the positive control. **represents Student’s t-test (unpaired, two-sided) p-value < 0.01, *represents Student’s t-test (unpaired, two-sided) p-value < 0.05. #represents Student’s t-test (unpaired, two-sided) p-value < 0.1 (marginally significant). (c) qPCR analysis of circadian miRNA primary transcripts of liver-specific Bmal1 cKO mice and wild-type mice. Per2 is used as the positive control. The y-axis has an exponential scale. Two-way ANOVA using circadian time (CT0 and CT12) and genotype (Bmal1 cKO vs. wild-type) as two factors were applied to assess the statistical significance. **represents ANOVA p-value for genotype < 0.01, *represents ANOVA p-value for genotype < 0.05, NS stands for not significant p-value.