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. 2016 Feb 22;6:21549. doi: 10.1038/srep21549

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(a) Western blotting analysis of suprabasin expression in ESCC cell lines stably expressing or silencing suprabasin. (b) Suprabasin promotes growth rate of ESCC cells, determined by MTT assay. (c) Representative micrographs (left) and quantification (right) of colonies formed by the indicated cells as determined by colony formation assays. (d) Representative micrographs (left) and quantification (right) of the BrdUrd incorporation assay in the indicated cells. All experiments were performed with KYSE510 and KYSE180 cells stably overexpressing or silencing suprabasin. Bars represent the mean ± SD of three independent experiments. *P < 0.05.