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. 2016 Feb 22;6:21651. doi: 10.1038/srep21651

Figure 1. Transcript levels of SGT1 in response to Phytophthora capsici infection and the transient overexpression of PcINF1/SRC2-1 in pepper leaves.

Figure 1

(a) The SGT1 transcript levels detected at different time points in pepper leaves inoculated with P. capsici. The 4-weeks-old pepper plants growing in the greenhouse were used for P. capsici infection. After P. capsici inoculation, the leaves next to the infected leaves of the pepper plants were harvested for RNA extraction at different time points. Pepper leaves treated with MgCl2 was used as a negative control (Mock). (b) The SGT1 transcript levels in pepper leaves transiently overexpressing the PcINF1/SRC2-1 fusion protein. Agrobacterial cells harboring the construct containing the PcINF1/SRC2-1 fusion vector or the empty vector (EV) were infiltrated into the leaves of the healthy pepper plants. 24 h and 48 h after Agro-infiltration, the Agro-infiltrated pepper leaves were collected for total RNA extraction. (a,b) Experiments were repeated with at least four independent biological repetitions. Error bars indicate standard deviation (SD). Asterisks indicate a significant difference using a Fisher’s protected least significant difference (LSD) test. *P < 0.05; **P < 0.01.