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. 2016 Feb 22;6:21651. doi: 10.1038/srep21651

Figure 10. The exclusive nuclear or cytoplasm localization of SGT1/SRC2-1 failed to trigger cell death response in pepper leaves.

Figure 10

(a) BiFC fluorescence and the cell death detection of NLS-(nls-) or NES-(nes-) fused SGT1/SRC2-1 combinations in N. benthamiana leaves 48 h post infiltration. GV3101 harboring the BiFC serial vectors were infiltrated into the leaves of N. benthamiana using a needleless syringe and maintained in the climate chamber, which were harvested and immersed in the DAPI solution and incubated in 37 °C for 1 h before fluorescence detection using the Leica laser scanning microscope. Meanwhile, the infiltrated pepper leaves were also harvested for trypan blue staining. Scale bars = 50 μm. (b) The electrolyte leakage measurement of pepper leaves infiltrated with GV3101 cells containing NLS-(nls-) or NES-(nes-) fused SGT1/SRC2-1 combinations. The infiltrated pepper leaves were harvested for electrolyte ion measurement. Scale bars = 100 μm. Data represents the mean ± SD from three independent biological repetitions. Different letters indicate statistically significant differences as analyzed with an LSD test (P < 0.05).