Figure 3. Transient co-expression of SGT1 and SRC2-1 induced cell death in pepper leaves.

(a) The DAB and trypan blue staining of the Agro-infiltrated pepper leaves. The DAB and trypan blue staining assay were performed at 48 h after infiltration with GV3101 cells harboring the constructs containing SGT1, SRC2-1, SGT1/SRC2-1, and EV (empty vector), respectively. (b) Electrolyte leakage in Agro-infiltrated pepper leaves at 24 and 48 hours post infiltration. The pepper leaves was infiltrated with GV3101 cells harboring constructs containing SGT1, SRC2-1, SGT1/SRC2-1, and EV (empty vector) grown to an OD₅₉₅ of 0.4, the infiltrated pepper leaves were collected at different time points for the electrolyte leakage measurement. (c) Quantitative real-time RT-PCR analysis of the transcript levels of stresses-associated marker genes in pepper leaves transiently expressing SGT1 and SRC2-1 at different time points after inoculation with P. capsici. Expression values are normalized by the expression level of CaActin and 18S ribosomal RNA. (b,c) Data represent mean ± SD from three independent biological repetitions. Different letters indicate statistically significant differences using an LSD test (P < 0.05).