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. 2016 Feb 22;6:21651. doi: 10.1038/srep21651

Figure 9. The artificial modulation of subcellular localization of SGT1 and SRC2-1 in cells of N. benthamiana leaves.

Figure 9

The NLS and NES were employed to artificially target SRC2-1 and SGT1 exclusively to the nuclei and cytoplasm, respectively. GV3101 cells containing the SRC2-1-GFP, NLS(nls)-SRC2-1-GFP, SGT1-GFP, NLS-SGT1-GFP, and NES (nes)-SGT1-GFP vectors were infiltrated into the leaves of N. benthamiana plants. 36 h post infiltration, the leaves were harvested and immersed in the DAPI solution and incubated in 37 °C for 1 h and covered with underslip and the fluorescent signals were detected using the Leica confocal laser scanning microscope. Scale bars = 50 μm.