Figure 7. CoFe₂O₄–2-NBDG NPs lead mesenchymal-like breast cancer cells to deep modification by hyperthermia.

(a) a MDA-MB-231 cell in a co-culture environment, where no MNPs have been previously administered. The IR-laser focusing for 4:30 minutes, on the position indicated by the red cross, did not evidence any effect, as it can be seen by comparing the cell before (a) and after (b) irradiation. Scale bars: 5 μm. For details see Supplementary Movie 8. (c,d) few MDA-MB-231 cells and a MCF7 cell in a co-culture environment, after the administration of CoFe₂O₄–2-NBDG NPs, before (c) and after (d) the hyperthermia treatment. In (d) a cell shape modification provoked by the localized heating, for 4:30 minutes, can be appreciated. The continuous wave IR-laser beam was focused on MNPs (indicated by the red cross). Scale bars: 20 μm. For details see Supplementary Movie 4.