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. 2016 Feb 22;6:21371. doi: 10.1038/srep21371

Figure 6. Microscopic imaging of whole excised FaDu tumors.

Figure 6

(a) Whole excised FaDu tumor and mouse tongue were imaged 90 minutes post-injection of PARPi-FL (75 nmol/167 μl PBS with 30% PEG300) using a custom-built dual-axis confocal microscope with 488-nm laser excitation. (b) Reconstruction of a Z-Stack spanning a depth of 250 μm to show PARPi-FL nuclear localization. (c) Whole excised FaDu tumors were also imaged using a commercial confocal laser endomicroscope featuring a flexible microprobe with a resolution of 1.4 μm. (d) FaDu tumor, mouse tongue, and muscle were imaged 90 minutes post-injection of PARPi-FL (75 nmol/167 μl PBS with 30% PEG300) following sacrifice of the animals. Images are representative frames within real-time video recordings of the organs at 488-nm laser excitation. Identical window/leveling has been applied in all images. (e) Analysis of signal intensity after PARPi-FL or vehicle injection. Ten frames per specimen were analyzed for their average pixel intensity using ImageJ (n = 3). Statistical significance was determined using an unpaired t-test, corrected for multiple comparisons by the Holm-Šidák method with an alpha of 0.05.