Figure 2.

(a) Partial gene cluster encoding the catabolism of sterols in M. neoaurum ATCC 25795. Genes in the map are color-coded according to assigned or proposed function: orange, mce cluster genes for steroids transportation; green, side-chain degradation genes; blue, nucleus cleavage genes; gray, unassigned function; yellow, gene encoding the transcriptional repressor KstR1. The numbers, e.g. 55 bp and 646 bp, beside the meander lines indicate the spaces between adjacent genes. (b) Schematic of the genomic organization of hsd4A homologues in M. neoaurum ATCC 25795 and other mycobacteria. Percentages, such as 71%, 81% and 67%, indicate the amino acid sequence identity of Hsd4A_MN in M. neoaurum ATCC 25795 with homologs from other mycobacteria, including M. tuberculosis H37Rv, M. smegmatis str. MC² 155 and R. jostii RHA1. The location of hsd4A is between the yrbE4B-4A and fadE26–27. The size and direction of genes are indicated by arrows with corresponding length to scale.