Figure 3. mESCs carrying the T (5:7) translocation retain ability to differentiate into three germ layers.

(a) T (5:7)-GFP-E14 cells cultured in normal conditions (with LIF). (b,c) T (5:7)-GFP-E14 cells cultured without LIF for 7 days. (d) T (5:7)-GFP-E14 retain the ability to differentiate into all three germ layers in vitro. All 3 groups of cells were differentiated for 7 days by removing LIF. The expression levels were analyzed by real-time PCR and compared with undifferentiated cells cultured in the medium with LIF. The expression levels were Lg transformed. Data are represented as the mean ± SD, n = 4. (e) Immunofluorescence staining of differentiated T (5:7)-mESCs with anti-Nestin (ectodermal markers), anti-Tuj1 (ectodermal markers), anti-Myosin (mesodermal marker) and anti-Gata4 (endodermal marker) antibodies. Nuclei were stained with DAPI (blue). Scale bars, 200 μm.