|
E. coli strain |
| WM3064 |
Donor strain for conjugation; ΔdapA
|
45 |
| C41(DE3) |
Recombinant protein expression host |
GE healthcare |
|
S. piezotolerans WP3 strains |
| WP3 |
Wild-type strain; GenBank accession no. CP000472
|
Laboratory stock |
| WP3Δ4RE |
Wild-type WP3 strain with 4 restriction enzymes gene deletions |
Laboratory stock (unpublished work) |
| WP3Δ4RE-WT |
WP3Δ4 strain harboring pSW2 |
This work |
| WP3Δ110–331 |
WP3Δ4 strain harboring pSW2Δ110-331 |
This work |
| WP3Δ464–641 |
WP3Δ4 strain harboring pSW2Δ464-641 |
This work |
| Plasmids |
| pRE112 |
Allelic-exchange vector |
46 |
| pET-24b |
His-tag protein expression vector |
Novagen |
| pET-24b-fpsR
|
pET-24b containing the coding region of fpsR gene |
This work |
| pSW2 |
Chl R, derivative from filamentous bacteriophage SW1 |
Laboratory stock |
| pSW2Δ110–331 |
pSW2 with deletion of the fragment between 110 and 331 in 5′UTR of fpsA gene |
This work |
| pSW2Δ464–641 |
pSW2 with deletion of the fragment between 464 and 641 in 5′UTR of fpsA gene |
This work |
