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. 2016 Feb 22;6:21913. doi: 10.1038/srep21913

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Copyright © 2016, Macmillan Publishers Limited

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(A) Procedure for measurement of platelet aggregation in the straight channel. Optical images were obtained by high-speed camera with time intervals (∆t) of 1 s. Scale bar indicates 100 μm. To distinguish adhered platelets from the flowing blood, a correlation map depicting the correlation coefficient of two consecutive images is obtained. By conducting additional image processing techniques, the area of adhered platelets (A_Platelet) is obtained. (B) Temporal variation of A_Platelet for a rat model with 30 days diabetic duration. Correlation maps at 10, 20, 30 min are inserted. (C) Variation of A_Platelet according to the duration of diabetes D_Diabetes. Typical binary images for the four groups are included. (*p < 0.005: significant difference from the control group).