Table 1.

Summary of culture conditions using mouse cells as a starting source

	Feeder cells	Supplements		Media	Duration	Functional SP?	Reference
0P9-DL
BM-HSPC	0P9-DL	IL-7	FIt3L	α-MEM + 20% FBS	5 weeks	Yes	Schmitt [6]
FL-HSPC	OP9-DL	IL-7	Flt3L	α-MEM + 20% FBS	5 weeks	Yes	Schmitt [6]
ESC	OP9 0P9-DL	IL7 IL-15	FIt3L	α-MEM + 20% FBS	3 weeks	Yes	Schmitt [39]
iPSC	OP9-DL	IL-7	FIt3L	α-MEM + 20% FBS	3 weeks	Yes	Lei [44]
pFib-DL
FL-HSPC	Primary fibroblast-DL	IL-7 SCF	Flt3L GW2580	α-MEM + 15% FBS	2 weeks	Yes	Mohtashami [25]
Dll-Fc
FL-HSPC	–	IL-7 SCF	FIt3L	RPMI + 10% FBS	2 weeks	Yes	Ikawa [33]

Abbreviations: BM-HSPC, bone marrow-derived hematopoietic stem progenitor cells; DL/Dll, delta-like ligand; ESC, embryonic stem cell; FBS, fetal bovine serum; FL-HSPC, fetal liver-derived hematopoietic stem progenitor cells; Flt3L, FMS-like tyrosine kinase 3 ligand; IL, interleukin; iPSC, induced pluripotent stem cell; α-MEM, minimum essential medium α; pFib, primary fibroblast; RPMI, Roswell Park Memorial Institute medium; SCF, stem cell factor; SP, single positive.