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. Author manuscript; available in PMC: 2016 Feb 22.
Published in final edited form as: J Neurosurg. 2012 Oct 12;117(6):1022–1031. doi: 10.3171/2012.9.JNS1212

Fig. 3.

Fig. 3

Ciliary neurotrophic factor receptor subunit–α is selectively expressed in undifferentiated tumor-initiating cells. Upper: Western blot analysis showing decreased CNTRFα (molecular weight 27 kD) expression in glioma-derived tumor-initiating cells with 1 μM RA treatment over 24 and 48 hours. The CNTRFα expression decreased with RA treatment over time. The β-actin was used as internal positive quantitative controls. Lower: Immunofluorescence staining for CNTRFα in 1-μM RA-treated tumor-initiating cells showing a marked decrease in CNTFRα expression. Cell nuclei are counterstained with DAPI, showing the cytoplasmic location of CNTRFα.