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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2015 Sep 16;14(12):2901–2908. doi: 10.1158/1535-7163.MCT-15-0217

Figure 4. IR effects on lactate production persist in the presence of hypoxia and correspond to relative radiosensitivity/resistance.

Figure 4

A) CoCl2 exposure mimics hypoxia chamber exposure as demonstrated by HIF-1α induction. B) Hypoxia (chamber, 1% O2) and CoCl2 exposure increase sensitivity to 2-DG in a clonogenic survival assay. C) IR exposure triggers a transient, reversible drop in cellular lactate levels. This effect is diminished by approximately 50% following exposure to CoCl2 for 24hr post-irradiation. All values normalized to corresponding control condition (1.0). D) Cells were exposed to increasing IR doses and effects of cell death ascertained using a clonogenic survival assay. 2-DG exposure radiosensitized tumors, while hypoxia (chamber, 1% O2) resulted in increased relative radioresistance. * indicates p-value < 0.05 compared to corresponding control condition. Each experiment was carried out at least in triplicate, with values indicating means and error bars representing standard deviation.