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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2015 Sep 16;14(12):2901–2908. doi: 10.1158/1535-7163.MCT-15-0217

Figure 5. Acute IR effects on reducing potential and lactate can be used to detect intrinsic radioresistance driven by loss of p53 activity.

Figure 5

A) HN30 (p53 wt) and HN31 (p53 mut) cells were exposed to IR (2 Gy) resulting in a transient, dose dependent, reversible (NAC) decrease in the NADH/NAD+ ratio. IR induced a proportionally larger decrease in cellular lactate levels in HN30 cells compared to their HN31 counterparts. B) HN30 and HN31 cells were exposed to IR (2 Gy) resulting in a transient, dose dependent, reversible (NAC) decrease in lactate levels. IR induced a proportionally larger decrease in cellular lactate levels in HN30 cells compared to their HN31 counterparts. C) IR (5 Gy) induced decreases in cellular lactate levels were blunted in HN31 cells compared to HN30 cells. D) IR induced decreases in cellular lactate levels were blunted in cells in which p53 activity was abrogated using shRNA (HN30shp53) compared to lentivirus transfected controls (HN30lenti). * indicates p-value < 0.05 compared to corresponding control condition. Each experiment was carried out at least in triplicate, with values indicating means and error bars representing standard deviation.