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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2015 Apr 2;84:254–262. doi: 10.1016/j.freeradbiomed.2015.03.021

Figure 4. Mitochondrial calcium uptake is increased with age and oxidative stress.

Figure 4

Representative fluorescent images of young, old and Sod1−/− LAL NMJs loaded with rhod-2 and stimulated for 2s with 30Hz and 100Hz stimulus trains (left). LAL presynaptic mitochondrial calcium levels were quantified for young, old, and Sod1−/− mice (right) and plotted as the background subtracted increases in rhod-2 fluorescence intensities at 30Hz and 100Hz stimuli. Error bars are SEMs.