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A) Single‐pixel‐based titration curves for cytosolic and nucleus regions of a single cell. Insets: False‐colored images of the free energy of unfolding at 37 °C, ΔG°u, calculated for each pixel. B) ΔG°u (37 °C) and C) T m single‐pixel histograms of the cytosol and nucleus of the same cell as shown in (A).
