Table 1.
Gene expression analysis of some key genes involved in protective defence mechanisms in leaves of drought- and salinity-stressed plants pre-treated with 10–8 M KM compared with respective stressed samples. The relative expression (fold change) of specific regulatory genes was determined by qRT-PCR in leaves of M. truncatula plants (values in bold letters indicate P<0.05, according to pairwise fixed reallocation randomization test). Microarray analysis expression values are also given for comparison purposes.
| Genes | Salinity | Drought | ||
|---|---|---|---|---|
| qRT-PCR | Microarrays | qRT-PCR | Microarrays | |
| Proteolysis genes | ||||
| Mt 7g 111 060 | −1.90 | −1.57 | −1.33 | 0.32 |
| Mt 7g 111 050 | −1.58 | −1.61 | −1.18 | 0.03 |
| Mt 4g 077470 | −1.15 | 0.83 | 1.45 | −1.51 |
| Mt 5g 061690 | 1.16 | 0.34 | −1.30 | −1.28 |
| Common genes | ||||
| Mt 1g 074950 | 1.51 | −1.70 | −7.75 | −4.90 |
| Mt 7g 093100 | 1.50 | 1.76 | −2.73 | −3.19 |
| Mt 3g 070860 | −2,09 | −1,53 | −1,62 | −1,79 |
| Mt 7g 024750 | −1,69 | −1,74 | 5,22 | 2,01 |