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Effect of Si on expression of OsNramp5 in the roots. Seedlings of WT (A) and lsi1 mutant (B) were exposed to 0.5 or 200 μM Mn without or with 1mM silicic acid for up to 3 d. The expression of OsNramp5 in the roots was determined by quantitative real-time PCR. Histone H3 was used as the internal standard. Expression relative to WT (0.5 μM Mn -Si) is shown. Data are means ±SD of three biological replicates. Different letters indicate a significant difference (P<0.05) using Duncan’s test.
