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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: Dev Cell. 2016 Feb 8;36(3):331–343. doi: 10.1016/j.devcel.2016.01.001

Figure 1. Cytosolic ALIX changes from closed to open conformation during M phase induction.

Figure 1

(A) Schematic illustration of the closed and open conformations of ALIX. (B) IB of IE and ME with MPM-2 and 3A9 after Ponceau S (Ponc) staining. (C-E) IP of IE and ME with the indicated antibodies, followed by IB with the indicated antibodies. (F) Incubation of IE and ME with GST or GST-Src, followed by IB of bound proteins with the indicated antibodies. (G) IB of IOE or MEE with MPM-2, and IP of IOE or MEE with the indicated antibodies, followed by IB with 1A3. (H) IB of IE, ME and CIP-treated ME with MPM-2. (I) ME was first incubated with GST or GST-CHMP4b at 4°C for 2 h, and then treated with CIP. IE, ME and the two samples of differently treated ME were immunoprecipitated with the indicated antibodies, followed by IB with the indicated antibodies. See also Figure S1.