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. 2015 Nov 11;15(1):289–304. doi: 10.1074/mcp.M115.054361

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 6. — Qualification of innate response proteins in R. conorii-infected HUVECs. A, qualification of IFN pathway. Shown are SID-selected reaction-monitoring (SRM)-MS measurements of STAT1 and ISG15 (bottom) for HUVEC stimulated with LPS (left) or infected with R. conorii (right). y axis is ratio of protein relative to internal SIS peptide (native/SIS) peptide (native/aqua). B, qualification of rickettsial proteins in Golgi membranes. SID-SRM-MS measurements for UvrABC system protein C are shown; putative ankyrin repeat protein RBE; and chaperone protein HtpG. Compared with LPS-stimulated cells, significant induction of each was observed. C, qualification of rickettsial proteins in soluble Golgi fraction. SID-SRM-MS measurements for human HLA proteins and the rickettsial proteins UVRVC and HPTG in the soluble Golgi fraction.