Fig. 4.

Neutralization of imipenem by recombinant AmpCs. The relative susceptibility of A. baumannii SK17-S to imipenem in the presence of recombinant AmpCs (WT, S90A, and S90D) were identified by the imipenem neutralization assay. The discs contained increasing amounts (4–7.5 μg) of recombinant proteins and imipenem (5 μg). Imipenem susceptibility was determined by measuring the diameters of the bacterial growth inhibition zones. All values were normalized to the mean value of the imipenem-only disc. Scale bars correspond to 10 mm.