Fig. 2.

Yeast two-hybrid analysis of interactions between circadian clock components. A, TOC1 interacts with ELF3 of the evening complex. Full-length ELF3, ELF4, and LUX preys were tested for association with TOC1, TIC and ELF4 baits (ELF3 and ELF4 were a positive control). B, The intermediate region of TOC1 interacts with the C-terminal portion of ELF3. ELF3 was divided into four based on conserved regions and tested for interaction with the 3 major regions of TOC1- the pseudoreceiver (PS), intermediate region (IR), and CONSTANS (CO), CO-like, TOC1 (CCT) domain. DBD = GAL4 DNA binding domain, AD = GAL4 activation domain. Empty = empty vector (just DBD or AD) Top panel: -l–W selects for presence of plasmids, bottom -l–W-H+2 mm 3AT selects for interaction. Assays were repeated twice with similar results.