Fig. 6.

Influence of siRNA knock-down of p38, HSP27 or vimentin on cytotoxicity of GA. A, Western blotting assay of levels of p38, HSP27 or vimentin in cells treated with siRNA against p38, HSP27 or vimentin. Cells treated with scrambled siRNA were used as negative control. B, Apoptosis of cells treated for 24 h by GA (at 2 or 4 μm) with or without pretreatment of siRNAs. siRNAs used were scrambled siRNA or siRNA against p38, HSP27 or vimentin. Apoptotic cells were quantified by supravital double staining with Annexin V-FITC and PI. Showed were representative results of three independent experiments. *p < 0.05 versus control cells. ‡p < 0.05 versus cells treated with 2 μm GA. #p < 0.05 versus cells treated with 4 μm GA.