Fig. 5.

Mitochondrial pyruvate carriers inhibition increases the infarct size after I/R in a mouse model. Langendorff-perfused mouse hearts were subjected to aerobic perfusion with modified Krebs solution in order to examine the toxicity of the mitochondrial pyruvate carrier inhibitor UK5099 (50 μm). The mouse hearts were subjected to 30 min of no-flow global ischemia followed by 120 min of reperfusion. Modified Krebs solution (Control) or Krebs solution with UK5099 (50 μm) was perfused for 120 min after ischemia. A, representative photographs of 2,3,5-triphenyltetrazolium chloride-stained mouse heart sections obtained after the I/R protocol and graph showing infarct size expressed as percentage of total ischemic area in each group. Cardiac function is presented as B. Left ventricular developed pressure (LVDP, mm Hg) (B) and left ventricular end diastolic pressure (LVEDP, mm Hg) (C) are shown. D, coronary flow (ml/min). Values are means ± S.E.; control group, n = 3. UK5099 group n = 4. * indicates p value <0.05.