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Tet2 is a de novo methylcytosine oxygenase: 75 nM [14C]-mCpG was duplexed to an unlabeled strand containing either CpG, mCpG, hmCpG, fCpG, or caCpG and then reacted with 5 μg/mL Tet2 for 10 min. TSA and the fraction of each ox-mC were measured by HPLC-LSC. Shown are the mean and SD from duplicate experiments.
