Table 1.
Summary of species-specific AFLP markers from three selective primer combinations. Diagnostic markers of S. multiplicata (Sm, in table) or S. bombifrons (Sb, in table) were identified as loci fixed in allopatric populations of one species, and absent in the other.
| Primer combination EcoRI/MseI | Sm fragment size (bp) | Sb fragment size (bp) |
|---|---|---|
| aac/cac | 63 | 104 |
| 121 | 108 | |
| 160 | 147 | |
| 247 | 153 | |
| 410 | 157 | |
| — | 276 | |
| — | 484 | |
| aag/caa | 93 | 68 |
| 107 | 84 | |
| 173 | 149 | |
| 284 | 163 | |
| 300 | 182 | |
| — | 325 | |
| — | 489 | |
| acg/cag | 158 | 214 |
| 189 | 496 | |
| Total species specific loci | 12 | 16 |