Fig. 4. The His₃Asp motifs of S100A12 are essential for antifungal activity against C. albicans. (A) Antifungal activity of 1000 μg mL^–1 S100A12 and S10012 ΔHis₃Asp (t = 20 h, mean ± SEM, n = 3). (B) Antifungal activity of 125 μg mL^–1 S100A12 and Zn(ii)–S100A12 (t = 30 h, mean ± SEM, n = 3). The Zn(ii)–S100A12 sample was prepared by pre-incubating S100A12 with 2 equiv. of Zn(ii) (11.9 μM) prior to the assay. The bar labeled “Zn(ii)” indicates the growth of a culture where 11.9 μM Zn(ii) was added to the YPD/Tris medium. For each assay, the YPD/Tris medium contained a ≈2 mM Ca(ii) supplement.