Table I.
Proteins associated with the membrane of EVs from adult F. hepatica. Figures represent number of unique peptides
| Protein | Identifier | Extract |
||
|---|---|---|---|---|
| Trypsin | NaHCO3 | TX100/SDS | ||
| Proteases & inhibitors | ||||
| Legumain 4/5 | BN1106 s1861B000097 | 7 | 12 | 0 |
| Cathepsin B | BN1106 s1772B000188 | 0 | 13 | 0 |
| Cathepsin A | BN1106 s1241B000264 | 3 | 7 | 0 |
| Cathepsin B6/8 | BN1106 s793B000177 | 2 | 5 | 0 |
| Cathepsin B fragment | BN1106 s8462B000006 | 0 | 4 | 0 |
| Cathepsin B4/5/7 | BN1106 s13444B000002 | 0 | 6 | 0 |
| Cathepsin L1 | BN1106 s8490B000026 | 0 | 5 | 0 |
| Lysosomal Pro-X carboxypeptidase | BN1106 s1620B000120 | 0 | 4 | 0 |
| M17 Leucine aminopeptidase 2 | BN1106 s617B000566 | 0 | 6 | 0 |
| 20S proteasome subunit alpha 6 | BN1106 s1579B000119 | 0 | 4 | 0 |
| Proteasome beta 1 subunit | BN1106 s5855B000168 | 0 | 4 | 0 |
| Proteasome subunit beta type-5 | BN1106 s6770B000051 | 0 | 5 | 0 |
| Proteasome subunit beta 2 | BN1106 s2242B000188 | 0 | 5 | 0 |
| Proteasome subunit alpha 7 | BN1106 s7720B000057 | 0 | 3 | 0 |
| Proteasome subunit alpha type | BN1106 s4981B000066 | 0 | 6 | 0 |
| Proteasome subunit alpha type | BN1106 s3658B000104 | 0 | 4 | 0 |
| 20S proteasome subunit alpha 3 | BN1106 s1639B000395 | 0 | 2 | 0 |
| Lysosomal pro-Xaa carboxypeptidase | BN1106 s3518B000132 | 2 | 3 | 0 |
| Proteasome subunit alpha 6 | BN1106 s1259B000205 | 0 | 3 | 0 |
| M17 Leucine aminopeptidase 1 | BN1106 s7079B000034 | 0 | 2 | 0 |
| 20S proteasome subunit beta 2 | BN1106 s2284B000154 | 0 | 4 | 0 |
| Cathepsin L1 | BN1106 s10332B000011 | 0 | 3 | 0 |
| Multicystatin (cys1) | BN1106 s1612B000138 | 0 | 2 | 0 |
| Proteasome subunit alpha type | BN1106 s9050B000016 | 0 | 2 | 0 |
| Pumps & transporters | ||||
| ABCB1 | BN1106 s274B000296 | 4 | 2 | 3 |
| MDR p-glycoprotein 1 | BN1106 s2471B000098 | 0 | 0 | 3 |
| Glucose-transporter | BN1106 s584B000350 | 0 | 0 | 2 |
| Phospholipid-translocating ATPase IIB | BN1106 s435B000242 | 0 | 0 | 3 |
| ATP synthase subunit alpha | BN1106 s4332B000087 | 0 | 2 | 0 |
| Metabolism | ||||
| Propionyl-CoA carboxylase alpha | BN1106 s1252B000359 | 17 | 3 | 0 |
| Glutamate dehydrogenase 1 NAD(P)+ | BN1106 s5767B000030 | 5 | 6 | 0 |
| Aldolase | BN1106 s4469B000065 | 2 | 2 | 0 |
| GAPDH | BN1106 s5174B000030 | 5 | 0 | 0 |
| Glutamine synthetase | BN1106 s2400B000186 | 3 | 3 | 0 |
| Propionyl-CoA carboxylase beta | BN1106 s1551B000468 | 0 | 3 | 0 |
| Malic enzyme | BN1106 s233B000262 | 6 | 0 | 0 |
| Hexokinase A | BN1106 s175B000200 | 0 | 0 | 2 |
| Retinol dehydrogenase | BN1106 s2277B000049 | 0 | 0 | 2 |
| Malate dehydrogenase | BN1106 s1459B000183 | 3 | 0 | 0 |
| Cytoskeleton | ||||
| Actin 5C | BN1106 s2907B000133 | 11 | 4 | 0 |
| Ezrin | BN1106 s1300B000145 | 2 | 0 | 2 |
| Fimbrin | BN1106 s1403B000129 | 7 | 2 | 0 |
| Alpha actinin | BN1106 s4069B000247 | 4 | 0 | 0 |
| Gelsolin | BN1106 s2349B000188 | 3 | 2 | 0 |
| Beta tubulin 1 | BN1106 s55B000372 | 3 | 0 | 0 |
| Rho1 GTPase | BN1106 s1908B000177 | 0 | 0 | 2 |
| Chaperones | ||||
| Ubiquitin-60S ribosomal protein L40 | BN1106 s6576B000103 | 0 | 2 | 2 |
| Hsp70 | BN1106 s309B000234 | 9 | 2 | 3 |
| Chaperonin containing tcp1 | BN1106 s1242B000159 | 2 | 0 | 0 |
| 14–3-3 protein | BN1106 s3904B000042 | 0 | 0 | 2 |
| Exosome biogenesis / vesicle trafficking | ||||
| Annexin | BN1106 s819B000364 | 8 | 4 | 0 |
| Tetraspanin-CD63 receptor | BN1106 s1657B000161 | 0 | 5 | 4 |
| Acid sphingomyelinase | BN1106 s1285B000159 | 2 | 6 | 0 |
| Annexin | BN1106 s945B000218 | 2 | 9 | 7 |
| Myoferlin | BN1106 s3585B000136 | 0 | 0 | 11 |
| Tetraspanin-1 | BN1106 s915B000136 | 0 | 2 | 2 |
| ALIX | BN1106 s2963B000136 | 0 | 0 | 6 |
| IST1 | BN1106 s3747B000112 | 2 | 0 | 7 |
| Otoferlin | BN1106 s3261B000048 | 0 | 0 | 7 |
| Vacuolar assembly/sorting protein D1D2-like protein | BN1106 s2316B000077 | 0 | 0 | 5 |
| ALIX | BN1106 s1871B000313 | 0 | 0 | 4 |
| Charged multivesicular body protein | BN1106 s2655B000264 | 0 | 0 | 4 |
| Vacuolar protein sorting-associated protein 4B | BN1106 s1437B000141 | 0 | 0 | 4 |
| Syntenin-1 | BN1106 s4740B000062 | 0 | 0 | 3 |
| Rab8 | BN1106 s258B000276 | 0 | 0 | 2 |
| CD63 antigen | BN1106 s4560B000072 | 0 | 2 | 0 |
| Charged multivesicular body protein 5 | BN1106 s6543B000070 | 0 | 0 | 5 |
| Vacuolar protein sorting 26 | BN1106 s1191B000313 | 0 | 0 | 2 |
| Vacuolar protein sorting-associated protein VTA1 | BN1106 s2858B000111 | 0 | 0 | 2 |
| Annexin B2 | BN1106 s500B000161 | 0 | 0 | 2 |
| Antioxidant/Defence | ||||
| Peroxiredoxin | BN1106 s1614B000280 | 0 | 3 | 2 |
| Secreted saposin-like protein SAP-3 | BN1106 s10326B000017 | 0 | 4 | 0 |
| Thioredoxin | BN1106 s4026B000080 | 0 | 3 | 2 |
| GST sigma class | BN1106 s1081B000242 | 0 | 2 | 0 |
| Carrier proteins | ||||
| Major vault protein | BN1106 s7273B000042 | 0 | 8 | 0 |
| Ferritin | BN1106 s3950B000041 | 0 | 3 | 5 |
| Niemann-Pick C2 protein | BN1106 s20469B000004 | 0 | 2 | 0 |
| Ferritin | BN1106 s709B000627 | 0 | 5 | 0 |
| Myoglobin | BN1106 s284B000287 | 0 | 3 | 2 |
| Enzymes | ||||
| Phosphodiesterase-nucleotide pyrophosphatase | BN1106 s1985B000403 | 0 | 4 | 3 |
| Carbonic anhydrase | BN1106 s3009B000044 | 0 | 3 | 3 |
| Alpha mannosidase | BN1106 s666B000200 | 0 | 3 | 0 |
| Ras-related protein Ral-A | BN1106 s637B000246 | 0 | 0 | 2 |
| Alpha-galactosidase | BN1106 s1241B000260 | 0 | 2 | 0 |
| Uncharacterized / other | ||||
| F. hepatica tegumental antigen-1 | AF153056 | 6 | 0 | 3 |
| DM9 domain-containing protein | BN1106 s5689B000026 | 2 | 4 | 4 |
| Calmodulin-like protein | BN1106 s678B000118 | 0 | 2 | 0 |
| Uncharacterized | BN1106 s3001B000132 | 0 | 5 | 5 |
| Uncharacterized | BN1106 s8038B000016 | 0 | 4 | 7 |
| Uncharacterized | BN1106 s4767B000028 | 0 | 2 | 3 |
| Uncharacterized | BN1106 s6821B000024 | 0 | 2 | 4 |
| Uncharacterized | BN1106 s440B000226 | 0 | 0 | 4 |
| Uncharacterized | BN1106 s1110B000106 | 0 | 2 | 2 |
| Uncharacterized | BN1106 s114B000615 | 0 | 2 | 2 |
| Uncharacterized | BN1106 s4767B000027 | 0 | 0 | 2 |
| Uncharacterized | BN1106 s4413B000122 | 0 | 4 | 0 |
| Uncharacterized | BN1106 s551B000321 | 0 | 0 | 3 |
| Uncharacterized | BN1106 s263B000609 | 0 | 4 | 0 |
| Uncharacterized | BN1106 s3381B000140 | 0 | 2 | 0 |
| Uncharacterized | BN1106 s739B000132 | 0 | 0 | 2 |