MS/MS fragmentation patterns recorded at the 20% HCD level from a sodiated bikunin CS-glycopeptide precursor ion, [M+3Na]3+ at m/z 1116.41.
A, The complete HCD spectrum. B, the m/z 180–370 region; C, the m/z 1000–1500 region; and D, the m/z 900–1000 region. All major ions are annotated to charge, m/z, Na+ content and glycan structure.