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. 2015 Sep 25;14(12):3118–3131. doi: 10.1074/mcp.M115.051136

Fig. 8.

Fig. 8.

MS/MS fragmentation spectra of H1 and H1+H2 Heavy chain peptides cross-linked to CS-chains hydrolyzed by chondroitinase ABC. The H1 C-terminal tryptic and semitryptic peptides LPDRVTGVDTD and VTGVDTD were identified cross-linked to ΔGlcAGalNAc at m/z 774.85 (A), to ΔGlcAGalNAcGlcAGalNAc at m/z 964.41 (B), to ΔGlcAGalNAcGlcAGalNAc at m/z 723.77 (C) and together with the C-terminal peptide VEND of H2 cross-linked to ΔGlcAGalNAcGlcAGalNAc at m/z 952.36 (D) and cross-linked to ΔGlcAGalNAcGlcAGalNAcGlcAGalNAc at m/z 1142.42 (E). Schematic structures of the corresponding precursor ions are shown in the box.