Fig. 7.

α-1-antitrypsin has reduced anti-elastase activity when bound to reconstituted HDL. Reconstituted HDL (rHDL) were prepared from apoA-I and phospholipids by cholate dialysis and then co-incubated with α-1-antitrypsin (A1AT) to generate A1AT enriched rHDL. A, Size exclusion chromatography on tandem Superdex 200 columns was used to isolate HDL bound A1AT from lipid free protein. B, The ability of lipid free and rHDL bound A1AT to inhibit neutrophil elastase (NE) activity was measured by fluorometric assay.