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. Author manuscript; available in PMC: 2016 Feb 22.
Published in final edited form as: Eur J Mass Spectrom (Chichester). 2015;21(3):201–210. doi: 10.1255/ejms.1338

Figure 5.

Figure 5

20 μm brain tissue sections from drug-treated and control mice were analyzed by MAIV operating at intermediate pressure. Solution of 0.5 μL 3-NBN/clozapine:clozapine-d8 mixture was spotted on the tissue section and the sample was loaded into the commercially available vacuum chamber (Scheme 2.A). The matrix sublimed in about 2 min. The ion intensities of clozapine and clozapine-d8 were averaged over the 2 min acquisition, and the intensity ratio was plotted against concentration ratio. Insets show the microscopy images of the matrix/clozapine:clozapine-d8 spotted on tissue section before and after MAIV analysis.