Table 1. Western blot detection conditions for MyD88 and Foxp3.

Target protein	Molecular weight (kD)	Sealing time and temperature	Primary antibody dilution	Primary antibody incubation time	Secondary antibody dilution	Secondary antibody incubation time
MyD88	35	2 h, room temperature	1:1000	overnight	1:100000	4 h
Foxp3	47	30 min, 4°C	1:2000	overnight	1:100000	4 h