Fig 4. The suppressive function of liver SSC^lowCD11b⁺Gr1^dim cells is dependent on a NO mechanism.

(A) Proliferation of carboxy-fluorescein diacetate, succinimidyl ester (CFSE)-labeled T cells cultured in the presence of Dynabeads mouse T-Activator CD3/CD28 along with purified liver SSC^lowCD11b⁺Gr1^dim cells. Different enzyme inhibitors (L-NIL, nor-NOHA, or catalase) were added at the start of each respective culture. (B) Nitrite levels were investigated in the culture supernatants after 60 h of co-culture (n = 3). (C) The intracellular iNOS expression was determined by flow cytometry. The percentage of positive cells is indicated. The data obtained from 3 separate experiments are shown. *P < 0.05 compared to T cells alone.