Fig 6. M-CSF is associated with SSC^lowCD11b⁺Gr1^dim cell expansion in the liver.

(A) Liver M-CSF mRNA was investigated by real-time RT-PCR (n = 5). (B) Liver M-CSF protein was investigated by immunohistochemistry (n = 5). Scale bars, 100 μm. (C) M-CSF mRNA in Hepa1-6 cells treated with fatty acid was analyzed by real-time RT-PCR (n = 5). (D) Bone marrow cells were cultured with M-CSF. The frequency of CD11b⁺Gr1^dimLy6C^high cells among the CD11b⁺Gr1^dim cells is shown. (E) The Ly6C^high and Ly6C^low populations (S1 Fig) were sorted and added to allogenic MLRs. *P < 0.05