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. 2016 Feb 22;11(2):e0149932. doi: 10.1371/journal.pone.0149932

Fig 7. Epi-illumination images of excised mouse lungs infected with tdTomato-expressing BCG or BCG with vector backbone.

Fig 7

(A) Representative images of excised lungs infected with 101 to 106 colony forming units (CFU) BCG17 (tdTomato expressing BCG strain) and 105 CFU BCG39 (BCG containing the same vector that does not express tdTomato (Vector)). (B) Correlation of fluorescence signal in ex vivo images of lungs and CFU in lung homogenates from the same animal. Error bars represent the standard error for each sample group. ** p‐value < 0.01: significantly different from fluorescence of vector control group (horizontal dashed line in B) calculated by non-parametric Kruskal-Wallis test with the Bonferroni posttest. All images and measurements represent tdTomato contribution to signal after spectral unmixing.