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. 2016 Feb 19;7:10845. doi: 10.1038/ncomms10845

Figure 2. CYP26B1 degrades endogenous RA in the developing testis.

Figure 2

(a) Urogenital ridges (UGRs, gonad plus mesonephros) dissected at 12.5 or 13.5 d.p.c. from RARE-lacZ transgenic embryos were cultured for 24 h in the presence of ketoconazole (0.7 μM) and stained to visualize RA signalling. (b,c) 12.5 d.p.c. UGRs from RARE-lacZ;Cyp26b1 wild-type (Wt) or RARE-lacZ;Cyp26b1 knockout (KO) embryos were stained to visualize RA signalling (b) or lacZ expression was measured by qRT–PCR of gonad-only tissue (c, n=22, 6, 20, 3). (d) To demonstrate that ectopic induction of Stra8 in the Cyp26b1-knockout testis requires RA signalling, 11.5 d.p.c. UGRs were dissected from Cyp26b1 wild-type (Wt) or knockout (KO) embryos and cultured for 50 h in the presence or absence of AGN193109 (AGN) and Stra8 expression was measured by qRT–PCR (n=6, 6, 7, 7, 3, 3, 2, 2). **P<0.01, ***P<0.001, ****P<0.0001 (Student's t-test, unpaired for c, paired for d, mean+s.e.m. shown). Scale bar, 200 μm.