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. 2016 Feb 19;7:10845. doi: 10.1038/ncomms10845

Figure 4. Meiotic onset is delayed in the absence of ALDH1A1.

Figure 4

(a) Gonads were collected from Aldh1a1-knockout (KO) or wild-type littermates (Wt) at 13.5 or 14.5 d.p.c. and subjected to qRT–PCR analysis for expression of the pre-meiotic gene Stra8 or meiotic genes Sycp3 and Dmc1 (n=5, 3, 4, 5 for 13.5 d.p.c. and 6, 8, 12, 15 for 14.5 d.p.c.). Meiotic onset was delayed at 13.5 d.p.c. but had largely recovered by 14.5 d.p.c. (b,c) Western blot analysis demonstrated that less SYCP3 protein was detectable in 14.5 d.p.c. gonad tissue of XX Aldh1a1-knockout (KO) compared with XX wild-type (Wt) littermates (n=3, 3, 3). NS, not statistically significant, *P<0.05, ****P<0.0001 (Student's unpaired t-test, mean±s.e.m. shown).