Skip to main content
. 2016 Feb 19;7:10789. doi: 10.1038/ncomms10789

Figure 10. Spacer and Zinc finger domains of YY1 are essential for inhibiting transactivation activity of Smad3 or Foxp3.

Figure 10

Transactivation activity of Smad3, Foxp3 or YY1 domain mutants at the promoters of the Foxp3, CD25 or Ctla4 genes was measured by transient reporter assay. EL4 cells were transfected with Foxp3 promoter-luc (a), Foxp3 promoter-CNS1-luc (b), CD25 promoter-luc (c) or Ctla4 promoter-luc (d) reporter construct in combination with Smad3D-, Foxp3- or YY1 domain mutant expression vectors. Error bars shown in a–d represent s.d. Statistical differences were analysed by Student's t-test (n=3). *P<0.05. **P<0.01. I, ionomycin; NT, no treatment; P, phorbol 12-myristate 13-acetate.