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. 2015 Mar 4;8(6):1201–1211. doi: 10.1038/mi.2015.11

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Copyright © 2015 Society for Mucosal Immunology

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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SMAR1 controls type 17 helper T cell (Th17) differentiation. (a) Gel shift assay on 350 bp of interleukin-17 (IL-17) matrix attachment region (MAR) using increasing concentrations of purified SMAR1 protein. Naive T cells were isolated and differentiated under Th17 condition for various time points (1, 3, 5, and 7 days). Chromatin immunoprecipitation (ChIP) experiments were done on the MAR region of IL-17 gene with antibodies against (b) SMAR1, (c) RNA Pol II, (d) HDAC1, (e) H3K4(me)3, and (f) acetyl-H3K9. ChIP assay with the naive (Th0) and Th2 differentiated cells to show the relative enrichment of (g) SMAR1, (h) HDAC1, and (i) H3KAc. Relative enrichment was calculated by subtracting the Ct value with the control IgGs in the real-time PCR assays.