Fig. 2.

Negatively correlated chemicals ameliorate mutant HTT fragment toxicity in PC12 cells. a Caspase activity measurements for chemical screening were carried out at 72 h post-HTT103Q-induction as caspase activity peaks at this time point. b All test plates contained a 10 μM ebselen treatment. Ebselen is known to prevent caspase 3/7 cleavage post HTT103Q induction, and therefore served as a positive control [31]. c Chemicals that induce gene expression changes that positively correlate with HD have no significant effect on caspase 3/7 activation in PC12 cells expressing HTT103Q. d Seven of the 12 selected chemicals that induce gene expression changes which were negatively correlated with HD reduce HTT103Q toxicity in PC12 cells. PC12 cells expressing HTT103Q were exposed to sub-cytotoxic concentrations of chemicals for 72 h. Mean ± SEM (N = 3). *P < 0.05, **P < 0.01, ***P < 0.001