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. 2016 Feb 22;11(2):e0149799. doi: 10.1371/journal.pone.0149799

Fig 5. Morphology and growth of human ASCs cultured in 3D collagen scaffolds over 21 days.

Fig 5

(A) Representative images captured from 3D collagen scaffolds seeded with human ASCs for 24 hours: haematoxylin and eosin (H&E) staining and scanning electron microscopy (SEM). The white arrow demonstrates human ASCs attached to the collagen scaffold. (B) Representative images of GFP-expressed human ASCs cultured in collagen scaffolds for 7 and 21 days. Scale bar = 200 μm. (C) The proliferation rate of GFP-expressed human ASCs cultured in 3D collagen scaffolds and on 2D collagen-coated plates (n = 3). **p<0.01 vs 2D culture by unpaired t-test.