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. 2016 Feb 22;11(2):e0149386. doi: 10.1371/journal.pone.0149386

Fig 3. Jujuboside B increased NO generation and stimulated eNOS activation by phosphorylating eNOS at Serine-1177.

Fig 3

(A)Jujuboside B increased NO generation in HAECs concentration-dependently. * P < 0.05, ** P < 0.01 compared with Control, n = 3. (B) Jujuboside B increased NO generation in HAECs negative time-dependently. * P < 0.05, ** P < 0.01 compared with 15 min group, n = 3. (C) L-NAME significantly inhibited Jujuboside B-induced NO generation. * P < 0.05, ** P < 0.01, n = 3. (D) L-NAME significantly inhibited Jujuboside B-induced eNOS activation. ** P < 0.01, ## P < 0.01, n = 4. (E) Western blot analysis of eNOS and p-eNOS at Serine-1177 in HAECs showed that Jujuboside B significantly increased p-eNOS Serine-1177 expression. (F) Densitometric analysis showed that Jujuboside B significantly increased p-eNOS Serine-1177 expression. * P < 0.05 compared with Control, n = 3.